Flash content link: https://vlab.amrita.edu/repo/BIOTECH/BIC/Titration_curves_of_%20amino_acids/index.swf

 

Materials Required:

 

1. 0.1M Hydrochloric acid
2. 0.1M Sodium Hydroxide
3. pH Meter
4. 0.1M  Glycine
5. Burette -2
6. Beaker
7. Stirrer
8. Standard Buffer of   pH=4, pH= 7, pH=10

 

Working steps:

 

1. Pipette out 20ml of the amino acid solution into a 100ml beaker.
2. Standardize the pH meter using the standard buffer solutions.
3. Determine the pH of the amino acid solution.
4. Add 0.3ml of 0.1M HCl from the burette and record the pH after each addition.
5. Continue adding the acid until the pH falls to 1.6
6. Wash thoroughly the pH electrode in distilled water.
7. Take 20 ml of amino acid solution in another beaker and check its pH.
8. Now titrate the amino acid solution by adding 0.3ml of 0.1M NaOH until the pH reaches 12.5.
9. Plot the titration curve using the values recorded and find the pKa values.
    

 Tabular Column:

    

 

 

 

 

 

 

 

 

 

 

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