 | An Overview of Virtual Proteomics Laboratory An Overview of Proteomics and our Team Member |
| Module I: An Overview of Gel-based Proteomics Module I: An Overview of Gel-based Proteomics |
| Experiment-1: Gel-based proteomics to analyze human serum proteome Objective: To compare the serum protein expression profiles of diseased patients having fever and healthy control samples using 2-D gel electrophoresis. |
| Experiment-2: Gel-based proteomics to analyze bacterial proteome Objective: To compare the profiles of drug treated and normal E. coli cultures grown under identical conditions using 2-D gel electrophoresis. |
| Experiment-3: Gel-based proteomics to analyze plant proteome Objective: To compare the protein expression profiles of plant leaves grow under conditions of drought (stress) and normal conditions using 2-D gel electrophoresis. |
| Module II: An Overview of MALDI-TOF MS Module II: An Overview of MALDI-TOF MS |
| Experiment-4: In-gel digestion of proteins for MS analysis Objective: To excise the spots from 2D or SDS-PAGE gels and perform in-gel trypsin digestion |
| Experiment-5: Sample preparation for the MALDI-TOF MS analysis Objective: To prepare the test and standard samples for the MALDI-TOF MS analysis |
| Experiment-6: MALDI-TOF instrumentation and analysis of serum proteins Objective: MALDI-TOF instrumentation and generation of peptide mass fingerprinting (PMF)from the serum proteins |
| Experiment-7: MS data analysis - Peptide Mass Fingerprinting (PMF) Objective: To analyze the MALDI-TOF peptide mass fingerprinting data |
| Experiment-8 : Molecular Weight Determination of Intact Protein Using MALDI TOF Determining the molecular weight of intact protein using MALDI TOF |
| Module III: An Overview of Bioinformatics Module III: An Overview of Bioinformatics |
| Experiment-9 : Sequence Alignment To perform Sequence Alignment |
| Experiment-10 : Homology Modelling Homology Modelling |
| Experiment-11 : Protein Function Annotation Protein Function Annotation |
| Experiment-12 : Molecular Docking Molecular Docking |
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