Molecular Biology Virtual Lab II

The study of biology at a molecular level. This field overlaps with other areas of biology and chemistry, particularly genetics and biochemistry. Molecular biology chiefly concerns itself with understanding the interactions between the various systems of a cell, including the interactions between DNA, RNA and protein biosynthesis as well as learning how these interactions are regulated.

Preparation of Equilibrated Phenol
Through this experiment you will able to understand the preparation of equilibrated phenol and its role in the biological/molecular experiments.
Isolation of RNA
To understand isolation of RNA, the most critical step in performing many fundamental molecular biology experiments.
Polyacrylamide Gel Electrophoresis
PAGE (Polyacrylamide Gel Electrophoresis) , is the most widely used analytical method to resolve separate components of a protein mixture based on their size.
Ligation ( Using T4 DNA Ligase)
Ligation ( Using T4 DNA Ligase)
Polymerase Chain Reaction (PCR)
Polymerase chain reaction is one of the technologies that not only made a tremendous impact on the scientific community, but also affected many aspects of our everyday lives.
Electroblotting
To familiarize the user with the technique of Electroblotting
Plating of the Bacteriophage
To demonstrate the ability of bacteriophage to replicate inside a susceptible host cell and to determine the concentration of phage particles in a suspension.
Plasmid Curing
Plasmids are extrachromasomally replicating molecules of DNA present in bacteria.Plasmid curing is a process of completely removing plasmids of bacteria by means of chemical agents such as Acriflavin ,acridine orange,SDS, etc Various methods involv
Extraction of Bacteriophage DNA from Large Scale Cultures Using Proteinase K and SDS
To extract genomic DNA from The bacteriophage In a lysate.
Preparation of stocks of bacteriophage lambda by plate lysis and elution
To prepare the stocks of bacteriophage in a laboratory.

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