Microbiology Virtual Lab II

To study the biochemical properties of microorganisms, the various techniques employed in cultivation of fungi and viruses along with the molecular level analysis of microbial genome.

Voges-Proskauer Test
The Voges-Proskauer test determines the capability of some organisms to produce non acidic or neutral end products, such as acetyl methyl carbinol, from the organic acids that result from glucose metabolism.
Triple Sugar Iron Agar
TSI test is designed to differentiate among the different groups or genera of the Enterobacteriaceae, which are all gram negative bacilli capable of fermenting sugars with the production of acid, and Hydrogen Sulphide
Urease Test
Urease Test determine the ability of microorganisms to degrade urea by means of the enzyme urease.
Litmus Milk Test
To introduce and demonstrate the principle and experimental set up for determining the microbe’s ability to transform milk substrate to metabolic end products
Slide Culture Technique for Fungi
To detail the experimental steps and protocol for culture , observation and identification of fungal mycelium and spores
Bacteriophage Plaque Assay for Phage Titer
To detail the experimental steps and protocol for the identification and quantification of bacteriophage plaques.
Isolation and Identification of Auxotrophic and Drug Resistant Mutants
To introduce and demonstrate the principle and experimental setup of the gradient plate technique replica plating technique for the isolation auxotrophic mutants
Isolation and Identification of Two Bacterial Unknowns
To introduce and demonstrate various steps involved in the identification of a bacterial unknowns from a mixed culture of organism.
Routes of Viral Inoculation in Embryonated Eggs
To study the different routes of inoculating virus in the embryonated eggs.
16S Ribosomal RNA Sequencing
To introduce and demonstrate basic principles and techniques behind the molecular level analysis of 16S Ribosomal RNA of bacteria.

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