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Restriction Digestion
1)Restriction enzymes are enzymes isolated from?

2)What is the normal role of restriction enzymes in bacterial cells?

3)Which type of restriction endonuclease cuts the DNA within the recognition site? 

4)Which enzymes recognize and cleave specific 4 to 8 base pair sequences?

5)Sticky ends are the result of

6)If a linear piece of DNA has two sites for BamHI restriction enzyme, into how many fragments will that restriction enzyme cut the DNA? 

7)Star activity of the Restriction Enzyme is due to

8)DNA sequence that restriction enzymes cut are mostly:

9)If a circular piece of DNA has four HindIII sites, into how many fragments will the DNA be cut? 

10)Bacterial cells protect their own DNA from degradation by restriction endonucleases


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