 | Gram Stain Technique The Gram stain is a differential staining technique used to classify & categorize bacteria into two major groups: Gram positive and Gram negative, based on the differences of the chemical and physical properties of the cell wall. |
| Aseptic Technique and the Transfer of Microorganisms Asepsis is the state of remaining free from pathogenic and contaminating microorganisms.This technique ensures a contaminant free environment while handling micro organisms. |
| Streak Plate Method The streak plate method is a rapid qualitative isolation method for obtaining discrete colonies from a mixed population. |
| Motility Test To introduce and demonstrate the principle and experimental set up for determining the motility of microbes |
| Catalase and Coagulase Test To introduce and demonstrate the principle and experimental set up for determining the microbe’s ability to detoxify hydrogen peroxide and /or to cause blood coagulation. |
| Selective and Differential Media for Identifying Microorganisms To introduce the preparation and use of different selective and differential media |
| Lecithinase Test To introduce and demonstrate the principle and experimental set up for determining the ability of microorganism to produce "Lecithinase" enzyme. |
| Bacterial Growth Curve To study the different phases of growth of a bacterium by plotting a curve with time of growth on the X-axis and optical density on the Y-axis. |
| Carbohydrate Fermentation Test To study the different carbohydrate utilization of bacteria using phenol red carbohydrate fermentation broth. |
| Differential and Cytological Staining Techniques To study the staining of bacteria with special stains that help to reveal their morphology thereby enhancing the contrast using a bright field microscope. |
| Antibiotic Susceptibility Testing To determine the susceptibility of a microbial species against different antibiotic agents. |
| Methylene Blue Reductase Test Checking the quality of the given milk sample |
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