|Preparation of Equilibrated Phenol|
Through this experiment you will able to understand the preparation of equilibrated phenol and its role in the biological/molecular experiments.
|Isolation of RNA|
To understand isolation of RNA, the most critical step in performing many fundamental molecular biology experiments.
|Polyacrylamide Gel Electrophoresis|
PAGE (Polyacrylamide Gel Electrophoresis) , is the most widely used analytical method to resolve separate components of a protein mixture based on their size.
|Ligation ( Using T4 DNA Ligase)|
Ligation ( Using T4 DNA Ligase)
|Polymerase Chain Reaction (PCR)|
Polymerase chain reaction is one of the technologies that not only made a tremendous impact on the scientific community, but also affected many aspects of our everyday lives.
To familiarize the user with the technique of Electroblotting
|Plating of the Bacteriophage|
To demonstrate the ability of bacteriophage to replicate inside a susceptible host cell and to determine the concentration of phage particles in a suspension.
Plasmids are extrachromasomally replicating molecules of DNA present in bacteria.Plasmid curing is a process of completely removing plasmids of bacteria by means of chemical agents such as Acriflavin ,acridine orange,SDS, etc Various methods involv
|Extraction of Bacteriophage DNA from Large Scale Cultures Using Proteinase K and SDS|
To extract genomic DNA from The bacteriophage In a lysate.
|Preparation of stocks of bacteriophage lambda by plate lysis and elution|
To prepare the stocks of bacteriophage in a laboratory.
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