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Biotechnology and Biomedical Engineering
Cell biology Virtual Lab I
Western blotting is a technique that .....
Sorts DNA fragement by size
Uses DNA probes to detect DNA fragements
Uses DNA probes to detect RNA fragements
Uses antibodies to detect proteins
Western blotting would be a useful technique to determine expression of a gene in a particular tissue.
After incubation of membrane with primary antibody, which buffer is used for washing the membrane?
The loading sample containing .......
Sample buffer, protein, and Milli-Q water
Sample buffer and protein
Sample buffer and Milli-Q water
Sample buffer only
The electrophoresis apparatus needs
Electrophoresis tank Buffer solution
power packs and electrodes
All the above
For verify protein transfer which stain is used?
Crystal violet staining
Gram's iodine staining
None of these
Primary antibody is diluted by using ........
5% non -fat dry milk
Secondary antibody is diluted by using......
5% non-fat dry milk
For the detection of protein in membrane which instrument is used?
Bio imaging sysyem
Gel documentation system
Assembly the immunblot sandwitch from cathode to anode. 1. Gel, 2. Filter paper, 3. Sponge, 4. PVDF membrane
Cite this Simulator:
vlab.amrita.edu,. (2012). Western Blotting. Retrieved 3 July 2022, from vlab.amrita.edu/?sub=3&brch=187&sim=1331&cnt=3
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