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Biotechnology and Biomedical Engineering
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Biological Image Analysis Virtual Lab
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Quantification of Amino Acids Present in a Mixture
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Quantification of Amino Acids Present in a Mixture
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Theory
Procedure
Self Evaluation
Simulator
Assignment
Reference
Feedback
1)
Quantification of spots in the thin layer chromatography gives
Random intensity of amino acids in the mixture.
Mean intensity of amino acids in the mixture
Relative intensity value of amino acids in the mixture
Amount of amino acids in the mixture
2)
Quantification of amino acids in the TLC relies on
Number of spots
Intensity of the spots
Side chains in the amino acids
Nature of the amino acids
3)
In TLC, the amino acids are detected using the reagent
Ninhydrin reagent
Barrit’s reagent
Barfoed’s reagent
Kovac’s reagent
4)
Which tool is used to find the area under the peak?
Wand tool
Line tool
Oval selection tool
Rectangular selection tool
5)
How can we label the peak percentage in the intensity plot of TLC?
Analyze --> Gels --> Re-plot Lanes
Analyze --> Gels --> Label Peaks
Analyze --> Gels -->Plot Next Lane
Analyze --> Gels --> Gel Analyzer Options
Cite this Simulator:
vlab.amrita.edu,. (2012). Quantification of Amino Acids Present in a Mixture. Retrieved 15 August 2022, from vlab.amrita.edu/?sub=3&brch=278&sim=1468&cnt=3
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