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Biotechnology and Biomedical Engineering
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Biochemistry Virtual Lab II
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Construction of Maltose Standard Curve by DNS Method
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Construction of Maltose Standard Curve by DNS Method
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Theory
Procedure
Self Evaluation
Animation
Assignment
Reference
Feedback
1)
In maltose, the glucose units are joined together by ………………….type of bond.
α(1,4)
β(1,4)
α(1,6)
β(1,6)
2)
If X is a non-reducing disaccharide of glucose, choose the appropriate glycosidic linkage in X from the list given below:
α(1,4)
α (1,2)
α(1,1)
α(1,3)
3)
In maltose standard curve experiment the absorbance was measured at:
210nm
350nm
540nm
610nm
4)
In the assay of reducing sugar by DNS, the DNS undergoes……………………….
Oxidation
Reduction
condensation
None of the above
5)
Which of the following is not a reducing sugar?
Glucose
Fructose
Sucrose
Maltose
6)
The DNS assay can be employed for estimation of following carbohydrates except
Glucose
lactose
Sucrose
maltose
7)
What is a reducing sugar?
A sugar which contains a ketone functional group that is readily reduced to an alcohol in basic solution.
A sugar that reduces other sugars in basic solution.
A sugar that is readily oxidized to a carboxylate ion in basic solution.
A sugar that contains an aldehyde functional group that is readily reduced to an alcohol in basic solution.
8)
The absorbance of a substance can be measured using which of the following?
Colorimeter.
Plate reader.
Both a & b.
Optical density reader.
9)
The color intensity of the reaction between maltose and DNS indicates:
Optical density.
Concentration of reducing sugar.
Both a & b.
None of the above.
10)
If the absorbance of the solution is zero, what does it imply?
100% transmittance.
Defect in colorimeter.
Concentration of solute in the solution is nil.
Both a & d.
11)
A standard curve means?
A curve which is linear.
A curve to determine the concentration of the unknown.
A curve of a standard compound.
All the above
12)
What is the role of sodium potassium tartarate tetrahydrate in the reaction?
To prevent destruction of sugar by dissolved oxygen in the solution.
To increase the amount of colour given by the sugar.
To prevent oxidation of sugar by dissolved oxygen.
All the above.
Cite this Simulator:
vlab.amrita.edu,. (2011). Construction of Maltose Standard Curve by DNS Method. Retrieved 17 February 2025, from vlab.amrita.edu/?sub=3&brch=64&sim=163&cnt=3
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