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Isolation of lymphocytes from whole blood




  • Can we isolate the lymphocytes from other sources (except whole blood) by this same procedure? Why?


  • Name some other anti coagulating agent used for the lymphocyte isolation procedure?


  • Explain the importance of  using the reagents used in this lymphocyte isolation procedure?


                         a)   Histopaque 1077 medium


                        b)      1X PBS Buffer


                        c)      Heparinised green top tube


                       d)      Freezing medium


                       e)  -80°C freezer



  • Can we isolate lymphocytes from clotted blood? Explain?


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