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Biotechnology and Biomedical Engineering
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Immunology Virtual Lab I
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SANDWICH Elisa
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SANDWICH Elisa
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Theory
Procedure
Self Evaluation
Animation
Assignment
Reference
Feedback
NPTEL Video
1)
A positive result from sandwich ELISA indicates that the patient's serum contains
Specific antibody
Specific antigen
Cytokines
None of the above
2)
An ELISA can be used for
quantitative analysis
size analysis
absorbance measurements
All of the above
3)
Which is not true for a blocking buffer solution ?
binds to all potential sites of nonspecific interaction
improves the sensitivity of an assay by reducing background signal
improves the signal-to-noise ratio
alters or obscures the epitope for antibody binding
4)
Washing step can be performed with a physiologic buffer such as Tris-buffered saline (TBS) or phosphate-buffered saline (PBS)
True
False
5)
The substrate of Horse radish peroxidase is
3,3’,5,5’-tetramethylbenzidine(TMB)
4-methylumbelliferyl phosphate(MUP)
p-nitrophenyl phosphate(NPP)
none of the above
Cite this Simulator:
vlab.amrita.edu,. (2011). SANDWICH Elisa. Retrieved 10 October 2024, from vlab.amrita.edu/?sub=3&brch=69&sim=699&cnt=3
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