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Biotechnology and Biomedical Engineering
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Immunology Virtual Lab I
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INDIRECT Elisa
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INDIRECT Elisa
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Theory
Procedure
Self Evaluation
Animation
Assignment
Reference
Feedback
NPTEL Video
1)
In indirect ELISA test, the order in which reagents are to be added is 1- enzyme-linked antibody 2- known antigen 3 - patient serum 4 - substrate
3- 2- 1- 4
2- 4 -1 -3
2 -3 -1 -4
4 -2 -1 -3
2)
The indirect ELISA requires known
Antigen
Antibody
enzyme
Patient sera
3)
Indirect ELISA detects the presence of antigen in patient sera
True
False
4)
The substrate of Horse radish peroxidase is
p-nitro phenyl phosphate (NPP)
4-methylumbelliferyl phosphate (MUP)
TMB
none of the above
5)
In the indirect ELISA test the enzyme-linked antibody will attach to
blocking buffer
variable region of the patient antibody
constant region of the patient antibody
patient antigen
Cite this Simulator:
vlab.amrita.edu,. (2011). INDIRECT Elisa. Retrieved 11 December 2024, from vlab.amrita.edu/?sub=3&brch=69&sim=721&cnt=3
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