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Biotechnology and Biomedical Engineering
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Immunology Virtual Lab II
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Fluorescent Labeling of Antibodies
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Fluorescent Labeling of Antibodies
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Theory
Procedure
Self Evaluation
Animation
Assignment
Reference
Feedback
1)
Antibodies can be labelled with FITC through which of the following residue :
Valine
Lysine
Histidine
Glycine
2)
Which is the buffer that we used in this experiment for the dialysis of antibodies:
PBS Buffer
Sodium bicarbonate Buffer
Sodium acetate Buffer
Sodium citrate Buffer
3)
Flourescent Dyes are :
Hydrophobic and bind non-covalently to proteins
Hydrophobic and bind covalently to proteins
Hydrophilic and bind non-covalently to proteins
Hydrophilic and covalently to proteins
4)
Absorbance used to determine the protein concentration in sample is :
210 nm
280 nm
600 nm
480 nm
5)
FITC dye mainly used for labelling which of the following biomolecules : 1. Antigen 2.Antibody 3.Amino acids 4.Lectins
1 only
2and 3 only
2 and 4 only
None of these
6)
The molecular weight of FITC dye was :
398
498
389
250
7)
Stoke effect is reffered to as :
The separation of the excitation wavelength maximum and the emission wavelength maximum.
The separation of the excitation wavelength maximum and the emission wavelength minimum.
The separation of the excitation wavelength minimum and the emission wavelength minimum.
The separation of the excitation wavelength minimum and the emission wavelength maximum.
8)
FITC dye have two isomers.ie Isomer I and isomer II. Isomer I was different from isomer II by :
Thiocyanate group on the 4th carbon of benzene ring
Thiocyanate group on the 3rd carbon of benzene ring
Thiocyanate group on the 5th carbon of benzene ring
Thiocyanate group on the 6th carbon of benzene ring
9)
Isomer II was different from isomer I by :
Thiocyanate group on the 4th carbon of benzene ring
Thiocyanate group on the 3rd carbon of benzene ring
Thiocyanate group on the 5th carbon of benzene ring
Thiocyanate group on the 6th carbon of benzene ring
10)
PBS Buffer is used because it is :
isotonic in nature
non-toxic to cells
Dilute the biomolecules
All the above
Cite this Simulator:
vlab.amrita.edu,. (2011). Fluorescent Labeling of Antibodies. Retrieved 11 February 2025, from vlab.amrita.edu/?sub=3&brch=70&sim=1060&cnt=3
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