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Gram Stain Technique
1)The Gram stain differentiates between bacteria is based on the composition of their _________ 

2)What happens when under decolourised? 

3)What happens when over decolourised? 

4)____________dissociates in aqueous solutions into CV+ and chloride (Cl – ) ions. 

5)What composes the thick cell wall of a gram positive bacteria? 

6)Which bacteria have an outer layer located outside the peptidoglycan layer? 

7)Which are the two sugars make up the peptidoglycan layer? 

8)Which bacteria has a thicker cell wall? 

9)Most bacteria have at least how many layers in their cell envelope? 

10)Which are the primary stain and counter stain used in Gram Staining 

11)Before you use your reagents to stain the bacteria, you need to heat fix your sample first by waving the slide through the blue flame on a Bunsen burner a few times. Why do you do this? 

12)Which is the crucial step in Gram Staining? 

13)Which of these factors will not affect your Gram stain results? (Given that everything else is done correctly.) 

14)What colour do Gram positive and Gram negative bacteria take; respectively? 

15)Role of mordant in Gram Staining 


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