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Biotechnology and Biomedical Engineering
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Molecular Biology Virtual Lab I
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Restriction Digestion
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Restriction Digestion
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Theory
Procedure
Self Evaluation
Animation
Simulator
Assignment
Reference
Feedback
1)
Restriction enzymes are enzymes isolated from?
Bacteria
Viruses
Humans
Mice
2)
What is the normal role of restriction enzymes in bacterial cells?
To degrade the bacterial chromosome into small pieces during replication.
To help in DNA replication.
To produce RNA primers for replication.
To degrade invading phage DNA.
3)
Which type of restriction endonuclease cuts the DNA within the recognition site?
Type I
Type II
Type III
All the above
4)
Which enzymes recognize and cleave specific 4 to 8 base pair sequences?
Ligases
Restriction endonucleases
Helicases
Polymerases
5)
Sticky ends are the result of
Treatment of a nucleotide sequence with DNA ligase
Cutting by restriction enzymes "off center" in a specific nucleotide sequence
Exposure of eukaryotic DNA to a prokaryotic plasmid
Infection of a cell with a bacteriophage
6)
If a linear piece of DNA has two sites for BamHI restriction enzyme, into how many fragments will that restriction enzyme cut the DNA?
3
4
2
1
7)
Star activity of the Restriction Enzyme is due to
Low ionic strength
High pH
High (> 5% v/v) glycerol concentrations
All the above
8)
DNA sequence that restriction enzymes cut are mostly:
Antiparallel
Palindromic
Symmetrical about the midpoint
Both b and c
9)
If a circular piece of DNA has four HindIII sites, into how many fragments will the DNA be cut?
5
4
3
6
10)
Bacterial cells protect their own DNA from degradation by restriction endonucleases
By deleting all recognition sites from the genome
By not producing any restriction endonucleases
By methylating the DNA at the sites that the enzyme recognizes
All the above
Cite this Simulator:
vlab.amrita.edu,. (2011). Restriction Digestion. Retrieved 17 February 2025, from vlab.amrita.edu/?sub=3&brch=77&sim=694&cnt=3
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