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Construction of Protein Standard Curve using Folin’s Lowry Method
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Assignment:

 

  1. Analyse the protein content in different samples using the above constructed protein standard curve.
  2. Find out the specific activity of beta amylase enzyme .
  3.  If 1ml of an unknown sample of protein is diluted to 10ml, and a  0.5ml portion of the diluted sample is found to contain 50ug of protein     when assayed for protein using the lowry method. What is the concentration of protein in ug/ml in the original sample ?
  4. If you mix two  unknown samples and repeat the lowry assay, is the absorbance equivalent to the sum of the two individual unknown samples that is  used
     

 

 

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