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Purification of IgG Antibodies using Affinity Chromatography
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1)In affinity chromatography the proteins are separated solely on basis of their : 
  
2)Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. 
  
3)Spacer arm is used 
  
4)--------------- can be employed as a ligand in the purification of glycoproteins. 
  
5)Protein A,protein G,protein L are derived from ------------- respectively. 
  
6)The binding of Antibodies to protein A occurs better at which condition: 
  
7)Chaotropes are used to denature the eluted proteins: 
  
8)The protein A and Protein B have a high affinity to the -----------------region of the Ig G type antibodies. 
  
9)Which of the following is used as a powerful tool for isolate and purify classes ,subclasses and fragments of immunoglobulins from biological fluids. 
  
10)The ligands are coupled to the solid support material by which type of interaction : 
  

  


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