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Purification of IgG Antibodies using Affinity Chromatography






1.Define the following terms:

              A. Spacer Arm
              B. Fraction Collector
              C. Ligand Coupling


2.Explain the basic principle of Affinity Chromatography?


3. What are  the different types of elutions?


4 Explain the importance of Protein A in the purification of antibodies by affinity Chromatography?


5. What is Chaotropes?










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