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Biotechnology and Biomedical Engineering
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Microbiology Virtual Lab II
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Isolation and Identification of Auxotrophic and Drug Resistant Mutants
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Isolation and Identification of Auxotrophic and Drug Resistant Mutants
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Theory
Procedure
Self Evaluation
Animation
Assignment
Reference
Feedback
1)
Mutants are unable to grow without one or more essential nutrients are termed as
Prototrophic
Auxotrophic
Oligotrophic
Phototrophic
None of the above
2)
Mutations which arise from high doses of chemical mutagen treatment or ionizing radiation exposures is termed as
Spontaneous mutations
Induced Mutations
Non induced mutations
Missense mutations
3)
Visual identification of auxotrophic mutants can be performed by
Gradient plate method
Mechanical filtration method
Pencillin selection method
Replica plate method
4)
An example of selective mutation from the group is
a mutant of Bacillus subtilis unable to form endospores.
a mutant of Escherichia coli unable to produce tryptophan
a mutant of Streptococcus pneumoniae that does not form capsules.
a mutant of E. coli that is resistant to streptomycin
5)
A common type of mutant which cannot synthesize an essential metabolite that wild-type cells produce is termed as
Nutritional heterotrophs
Nutritional auxotrophs
Nutritional prototrophs
Nutritional Phototrophs
6)
Type of mutation in which a change in code leads to substitution of a different amino acid is called a ............
Missense mutation
Non sense mutation
Point mutation
Silent mutation
7)
A researcher picks an E.coli colony from a Streptomycin gradient plate and inoculated on to another Streptomycin gradient plate. After incubation there is no growth on the on the HSC region. The reason is
Lack of sufficient inoculums.
Problems in the gradient plate preparation.
The selected E.coli colony is resistant mutant of Streptomycin.
The selected E.coli colony is Streptomycin sensitive.
8)
The significance of overlay technique in Gradient plate technique is :
Nutrient agar sets easily compared to Streptomycin agar.
Streptomycin diffuses into the lower layer, producing a gradient of antibiotic concentration from low to high.
Growth rate is more in Streptomycin agar than in nutrient agar.
None of the above.
9)
Absence of microbial growth on the HSC region of gradient plate indicates:
Absence of Streptomycin resistant mutants.
Presence of Streptomycin sensitive colonies.
Lack of inoculums.
Both a and c
10)
The importance of replica plating method in the isolation of auxotrophic mutants is
It is a positive selection method.
It is a negative selection method.
It is a spontaneous selection method.
None of the above.
11)
A point mutation that changes a codon specifying an amino acid into a stop codon is
Frame shift mutation
Nonsense mutation
Missense mutation.
Silent mutation.
12)
Mutations may alter the organisms
Morphology
Nutritional or biochemical properties
Biosynthetic pathways
All the above
13)
Antibiotics used for the isolation of Gram negative bacteria except
Streptomycin
Erythromycin
Rifampicin
Ergosterol.
Cite this Simulator:
vlab.amrita.edu,. (2011). Isolation and Identification of Auxotrophic and Drug Resistant Mutants. Retrieved 11 December 2024, from vlab.amrita.edu/?sub=3&brch=76&sim=1089&cnt=3
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