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Triple Sugar Iron Agar


Student assignment:


  1. You are provided with a culture tube of P. aeruginosa, E. coli, Salmonella typhimurium , Shigella flexneri. What biochemical test is more accurate to differentiate them?
  2.  Name the acid base indicator in the TSI test and the colour change for positive reaction. Explain the principle behind TSI test?
  3. Explain what happens if
          a) only glucose is fermented
          b) lactose is fermented or
          c) no fermentation occurred in a TSI culture tube

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