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Biotechnology and Biomedical Engineering
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Molecular Biology Virtual Lab I
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Transformation of the Host Cells
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Transformation of the Host Cells
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Theory
Procedure
Self Evaluation
Animation
Assignment
Reference
Feedback
1)
During transformation,
Foreign DNA is inserted into the plasmid
A cell incorporates DNA from outside the cell
A prokaryote is changed into a eukaryote
None of the above
2)
To demonstrate transformation in the lab, you would need
Insert DNA and recipient cells
Recipient DNA and DNA ligase
Male and female cells
None of the above
3)
White colonies indicates
Plasmid
Non –transformed cells
Insert DNA only
Transformed cells
4)
E.coli DH5α is resistant to
Streptomycin
Kanamycin
Ampicillin
Ciprofloxacin
5)
Which is the enzyme that cleaves the X-gal?
β- Galactosidase
Permease
Transacetylase
Glucosidase
6)
Temperature used in the CaCl2 based transformation method,
41°C
38 °C
42 °C
52 °C
7)
IPTG is a
Metabolisable substrate
Gratuitous inducer
Chromogenic substrate
Detergent
Cite this Simulator:
vlab.amrita.edu,. (2011). Transformation of the Host Cells. Retrieved 23 March 2025, from vlab.amrita.edu/?sub=3&brch=77&sim=1107&cnt=3
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