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Ligation ( Using T4 DNA Ligase)
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1)In molecular biology, DNA ligase is a special type of ligase that can link together two ________ strands that have double-stranded break 
  
2)The leaving group in an E. coli DNA ligase mediated reaction is 
  
3)DNA ligases are used with ___________ to insert DNA fragments into plasmids 
  
4)The common commercially available DNA ligases were originally discovered in bacteriophage ________ 
  
5)If the ambient temperature exceeds Tm, homologous pairing of the sticky ends will not occur because the high temperature disrupts ________
  
6)A DNA strand has the following bases: G T C A G C A. What are the bases on its complimentary strand? 
  
7)T4 DNA ligase is similar to E. coli DNA ligase, except that ATP is a cofactor rather than NAD. Besides ligated DNA, another product is 
  
8)In order to produce a DNA fragment that can be inserted into the DNA of a second organism one would need to 
  
9)Cuts in DNA are sealed with 
  
10)Sticky ends are created by 
  
11)The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is 
  
12)Vectors are
  

  


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