Ligation ( Using T4 DNA Ligase)

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Students' Assignment:-

What are some potential problems with the ligation reaction using T4 DNA Ligase that can lead to transformation failure?
What problems can be encountered in the restriction digest that can cause ligation using T4 DNA Ligase or subsequent transformation to fail?
What controls should be run to test the cells and DNA when using T4 DNA Ligase?
When should T4 DNA Ligase be the enzyme of choice?

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