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| . | Quantification of Stained Liver Cells |
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| . | For informations about installation of the image analysis tool, access the “Simulator†tab Step 1: Open ImageJ by double clicking ImageJ icon from the desktop or one may go to start programme ImageJ on the windows operating system. The image will open in a new window as shown below
 Step 2: To analyze an Image into ImageJ, we have to import the image into ImageJ. To perform that, Go to files ---> Open. A popup window will appear. Use Choose button to choose your respective images into ImageJ software. The imported picture will shown in a new window
 Step 3: Convert the images into "grayscale†(go to image ---> Type ---> RGB stack).
 Step 4: Adjust the RGB grey scale manually by moving the lower slider
 Step 5: Use the Image ---> Stacks ---> Make Montage command to view all three channels at the same time.
  Step 6: Adjust the Image ---> Adjust ---> Threshold tool. The threshold must be manually adjusted by moving the lower slider until the stained liver tissue is highlighted in red.  Step 7: Set threshold level and press “OK† Step 8: Choose AnalyzeSet Measurements. The Set Measurements window opens. Select Area, Area fraction, Limit to Threshold and Display label measurements. Then click OK.
 Step 9: To measure the selected area. Press Analyze ---> Measure. The Area and mean area of the stained samples appears in a new window. Repeat the above steps until all bands have been measured. Then copy all the values to a spreadsheet. The quantified images are now ready for data analysis in the spreadsheet program.
 Result Interpretation:
“Results†window indicates the Area and Percentage area of the stained cells. Here 23.2555% of the area contains the stained cells. Cite this Simulator: |
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