The animation is self-illustrative. Instructors may choose to use the interactive version in class before detailing the protocol for brain slice preparation. Typically this approach suits any brain slicing procedure however will fit in detail to ventral brain slice preparation.
Click on the simulator window to load the animation. Participate in a full lab to go through the various stages to perform a brain slice.
You will have to click/move using the computer mouse to pass through various stages of the experiment.
Observe the size of beakers and quantity of anesthesia. Rats may die if anaesthetized for a long period. If you are unsure about the various steps, please repeat the experiment until you are familiar with all details.