Home
Project
Workshop
Nodal Centres
Apply for Nodal Centre Program
Nodal Centre List
Free online demo
Nodal Centre Inaugurations
Unique Login ID
News & Events
Publications
Survey
Faculty Survey
Student Survey
Contact us
Login
.
you are here->
home
->
Biotechnology and Biomedical Engineering
->
Biochemistry Virtual Lab II
->
Effect of Substrate Concentration on Enzyme Kinetics
.
.
Effect of Substrate Concentration on Enzyme Kinetics
.
.
Theory
Procedure
Self Evaluation
Animation
Assignment
Reference
Feedback
NPTEL Video
1)
Which of the following parameters does not influence enzyme activity?
pH
Temperature
Product degradation
Effectors
Substrate concentration
2)
What is Km in Michaelis – Menten equation?
Michaelis- Menten constant.
Substrate.
Substrate concentration.
Enzyme activity.
Both a and c.
3)
When V0 is plotted against [S], why is it not possible to determine Vmax?
V is dependent of S at higher substrate concentrations.
Gradual upward slope of the hyperbolic curve at high substrate concentrations.
Km does not change, but Vmax is lowered.
Increase availability of enzyme by expression of more enzyme protein.
4)
Y intercept of LB equation:
1/Vmax
-1/KM
1/KM
1/[S]
5)
For an enzyme that follows Michaelis – Menten kinetics, Km is equal to:
The [S] at one half of Vmax.
The V at one half of Vmax.
The [S] at one half of V.
Two times the Vmax.
6)
What is a double reciprocal plot also called?
Michaelis – Menten plot
Line plot
Lineweaver – Burk plot
Inverse plot
Cite this Simulator:
vlab.amrita.edu,. (2011). Effect of Substrate Concentration on Enzyme Kinetics. Retrieved 22 March 2025, from vlab.amrita.edu/?sub=3&brch=64&sim=1090&cnt=3
.....
.....
.....
Copyright @ 2025 Under the NME ICT initiative of MHRD
Powered by
Amrita
Virtual Lab Collaborative Platform
[ Ver 00.13. ]