Flash content link: https://vlab.amrita.edu/repo/BIOTECH/BIC/Titration_curves_of_%20amino_acids/index.swf
Materials Required:
- 0.1M Hydrochloric acid
- 0.1M Sodium Hydroxide
- pH Meter
- 0.1M Glycine
- Burette -2
- Beaker
- Stirrer
- Standard Buffer of pH=4, pH= 7, pH=10
Working steps:
- Pipette out 20ml of the amino acid solution into a 100ml beaker.
- Standardize the pH meter using the standard buffer solutions.
- Determine the pH of the amino acid solution.
- Add 0.3ml of 0.1M HCl from the burette and record the pH after each addition.
- Continue adding the acid until the pH falls to 1.6
- Wash thoroughly the pH electrode in distilled water.
- Take 20 ml of amino acid solution in another beaker and check its pH.
- Now titrate the amino acid solution by adding 0.3ml of 0.1M NaOH until the pH reaches 12.5.
- Plot the titration curve using the values recorded and find the pKa values.
Tabular Column:

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